FSL 818 - Transporte em membranas e epitélios -M/DFSL 818 TRANSPORTE EM MEMBRANAS E EPITÉLIOS (02-00) 02-30
Prof. Dr. Bernardo Baldisserotto
OBJETIVOS:
Propiciar informações sobre transporte de substâncias em membranas e epitélios, bem como métodos de estudo dos mesmos e a ação de vários fármacos sobre esses sistemas de transporte.
EMENTA:
Canais de membrana, sistemas de transportadores, métodos de estudo de canais de membrana e transportadores, ação de diversas substâncias sobre os canais e sistemas de transporte.
METODOLOGIA E/OU INSTRUMENTOS DE ENSINO:
Seminário individual, discussão de artigos científicos, aulas expositivas, quadro, equipamento audiovisual (data show).
FORMA DE AVALIAÇÃO:
Seminários, participação em aulas.
PROGRAMA
UNIDADE 1 – Estrutura da membrana e epitélios transportadores
1.1 – Membrana plasmática
1.2 – Epitélios transportadores: membranas apical e basolateral, vias transcelular e paracelular
UNIDADE 2 – Canais
1.1 – Aquaporinas
1.2 – Canais iônicos
UNIDADE 3 - Transportadores
1.1 – Sistemas de transporte ativo (Na+,K+-ATPase, Ca2+-ATPase, K+,H+-ATPase)
1.2 – Simporte e antiporte
UNIDADE 4 – Técnicas de estudo de canais iônicos e epitélios
4.1 – Patch clamp
4.2 - Radioisótopos
4.3 – Câmara de Ussing - corrente de curto circuito
4.4 – Cultura de células
4.5 – Bloqueadores de canais e sistemas de transporte
4.6 – Biologia molecular: uso de animais com genes “nocauteados”
BIBLIOGRAFIA:
Na internet: Transport Classification Database (www.tcdb.org)
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